Journal: Journal of Histochemistry and Cytochemistry

Article Title: Localization of Angiopoietin-1 and Tie2 Immunoreactivity in Rodent Ependyma and Adjacent Blood Vessels Suggests Functional Relationships

doi: 10.1369/jhc.2009.954610

Figure Lengend Snippet: Primary antibodies and lectin used in tissue section labeling, immunoprecipitation, and Western blotting

Article Snippet: Tie2 , Santa Cruz Biotechnology , sc-9062 , Rabbit , Internal aa 234–533 (human) , 1:25 , Cy3.

Techniques: Labeling, Immunoprecipitation, Western Blot, Immunolabeling, Amplification, Plasmid Preparation

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Localization of Angiopoietin-1 and Tie2 Immunoreactivity in Rodent Ependyma and Adjacent Blood Vessels Suggests Functional Relationships

doi: 10.1369/jhc.2009.954610

Figure Lengend Snippet: Relationships of ependymal and vascular Angpt1 IR and tyrosine kinase 2 (Tie2) IR in normal adult female rats (confocal microscopy). (A) Angpt1 IR in aqueductal ependyma; (B) tomato lectin (T. Lect.)–labeled ependyma and a peri-ependymal blood vessel (bv); (C) merge of A,B and Hoechst nuclear stain (blue color) showing Angpt1-immunoreactive processes between the ependyma and an adjacent blood vessel. (D) Angpt1 IR in aqueductal ependyma; (E) Tie2 IR in a membranous pattern in the ependymal layer and in an adjacent blood vessel (bv). Insert: digital magnification of a portion of the ependyma showing concentration of Tie2 IR (red) on the lumenal surface and between adjacent ependymal cells in a Hoechst (blue)-stained section; (F) merge of D,E and Hoechst nuclear stain (blue color) showing Angpt1-immunoreactive processes (arrow) directed toward the Tie2-immunoreactive blood vessel (bv) and close association of membranous Tie2 IR and cytoplasmic Angpt1 IR in the cuboidal ependyma. (G) Angpt1-immunoreactive process; (H) Tie2 IR in a peri-ependymal blood vessel (bv); (I) merge of G,H and Hoechst nuclear stain (blue color) showing the close relationship between the Angpt1-immunoreactive process (arrow) and the Tie2-immunoreactive blood vessel (bv). (J) Angpt1 IR in a blood vessel; (K) Tie2 IR (arrow); (L) merge of J,K and Hoechst nuclear stain (blue color) showing the association between vascular Angpt1 IR and Tie2 IR. Note: Panels E and F were digitally postprocessed by linearly attenuating the intensity of the red signal using the “Image-Adjust-Levels” command in Adobe Photoshop software (Adobe Systems). Asterisk indicates aqueduct lumen. Bars: A–F = 25 μm; G–L = 10 μm.

Article Snippet: Tie2 , Santa Cruz Biotechnology , sc-9062 , Rabbit , Internal aa 234–533 (human) , 1:25 , Cy3.

Techniques: Confocal Microscopy, Labeling, Staining, Concentration Assay, Software